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Plasmid#162373PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8f
UseTags6xHisExpressionMammalianMutationQ315LPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8s
Plasmid#162371PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8s
UseTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag
Plasmid#181814PurposeExpresses murine Muc5b D1 with cleavable His tag, residues 50-426DepositorInsertMuc5b D1 (Muc5b Mouse)
UseTagsTEV cleavage site followed by 6xHistidine tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag
Plasmid#187994PurposeExpresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cellsDepositorInsertmucin2 D1D2D'D3 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D' with cleavable His tag
Plasmid#187993PurposeExpresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cellsDepositorInsertMucin2 D1D2D' (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p1585588
Plasmid#62620PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
dihydroflavonol 4-reductase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1_mouseST6galI
Plasmid#178435Purposemurine St6gal1 expression plasmidDepositorInsertSt6gal1 (St6gal1 Mouse)
UseTags6xHIS-TEV Cleavage SiteExpressionMammalianMutationCoding sequnce for amino acids 85-403PromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10
Plasmid#162387PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mEmerald Rab7a
Plasmid#115238PurposeTo express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.DepositorInsertRab7a fused to mEmerald (RAB7A Human)
UseAAVTagsmEmeraldExpressionMammalianMutation7 Amino Acids (21 Nucleotides) between mEmerald a…PromoterHuman UbCAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10
Plasmid#162388PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
UseTags6xHisExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10
Plasmid#162386PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
UseTags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEXneo-TrkC
Plasmid#190202PurposeTo express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.DepositorInsertTrkC (NTRK3 Human)
UseEucaryoticTagsExpressionMammalianMutationPromoterMoloney mouse sarcoma virus LTRAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10
Plasmid#162383PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
UseTags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEXneo-TrkB
Plasmid#190203PurposeTo express the Human TrkB receptor under the Moloney mouse sarcoma virus LTRDepositorInsertTrkB (NTRK2 Human)
UseTagsExpressionMammalianMutationPromoterMoloney mouse sarcoma virus right LTRAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-mGreenLantern-KASH
Plasmid#191094PurposeTo express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertmGreenLantern-KASH
UseAAV and Synthetic BiologyTagsmGreenLantern fused to the KASH domain of Nesprin…ExpressionMammalianMutationOptimizzation was done using the Human codon usagePromoterCMV enhancer and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only