pcdna3.1_mouseST6galI
(Plasmid #178435)

• Purpose: murine St6gal1 expression plasmid
• Depositing Lab: Deborah Fass
• Publication: Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.15252/embj.2022111869.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: St6gal1
• Alt name: ST6 Beta-Galactoside Alpha-2,6-Sialyltransferase 1
• Species: M. musculus (mouse)
• Mutation: Coding sequnce for amino acids 85-403
• GenBank ID: 20440 NP_001239434.1
• Entrez Gene: St6gal1 (a.k.a. AW742324, Siat1, St6Gal-I, St6gal, St6galI)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHIS-TEV Cleavage Site (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GAATCTCTATTTCCAGGGAGGTAAGCCACAGCCCTCCTTGCAGG
• 3′ sequencing primer: GTGCTGGATATCTGCAGAATTCTCAACAGCGATTGTTCCGGAAGCC

Resource Information

• Supplemental Documents:
  • Pl122 ST6GAL1 pcDNA QSOX1-HIS-TEV-ST6.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry