-
Plasmid#187150Purposemammalian cell expression of FLAG-HUWE1 L2976A/P2977ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationL2976A/P2977APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UIM-mut
Plasmid#187148Purposemammalian cell expression of FLAG-HUWE1 A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationA1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA-mut
Plasmid#187146Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330APromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3
Plasmid#100781PurposeMammalian expression of BNIP3DepositorInserthuman-BNIP3 (BNIP3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-CCR5
Plasmid#98950PurposeMammalian expression plasmid for N-terminal HA-tagged human CCR5DepositorInsertCCR5 (CCR5 Human)
UseTagsHA (YPYDVPDYA) epitope tag and Signal/leader sequ…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
8xNFAT-ZsG-hCD4
Plasmid#162746PurposeNFAT-driven ZsGreen-1 reporter gene with human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_SNCA:EGFP
Plasmid#92195PurposeLentiviral overexpression vector to make stable bicistronic cell line for α-Syn screenDepositorInsertDsRed-IRES-SNCA-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CXCR4
Plasmid#98944PurposeMammalian expression plasmid for untagged human CXCR4DepositorInsertCXCR4 (CXCR4 Human)
UseTagsExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
VP-FKBP51
Plasmid#89097Purposemammalian expression of FKBP5 also referred to as FKBP51 with N-terminal VP16 activation domainDepositorInsertFKBP5 (FKBP5 Human)
UseTagsFLAG and VP16ExpressionMammalianMutationPromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-DEST
Plasmid#119745PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA PSPC1
Plasmid#101764PurposeExpresses PSPC1 in mammalian cellsDepositorInsertparaspeckle component 1 (PSPC1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-TM
Plasmid#111844PurposeMammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tetherDepositorInsertHIV-1 Env (BG505 SOSIP.664)
UseTagsCD5 leader sequence and TM helix from MHC class IExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IKZF1-V2-V5
Plasmid#107390PurposeMammalian expression of IKZF1-V2-V5DepositorInsertIKZF1-V2 (IKZF1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorArticleInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
STIM1-EGFP
Plasmid#210370PurposeExpresses STIM1 fused with EGFP in mammalian cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD1-EGFP
Plasmid#131206Purposemammalian expression, tet RDepositorInsertNOD1 (NOD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-ZMYND11
Plasmid#65402PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertZMYND11 (ZMYND11 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only