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Plasmid#117170PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLSDepositorInsertreci_wtCas9 with SV40 NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVV613
Plasmid#108281PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM-ST1!TB
Plasmid#48654PurposeBacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicolDepositorInsertBacterial ST1 crRNA to prototspacer B
UseCRISPRTagsExpressionMutationPromoterJ23100 promoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSLT
Plasmid#51498PurposepLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmidDepositorInsertsT30 TALEN pair
us:NPTII repair template
UsePlant t-dna plasmidTagsExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-HT-Cbx7-Delta-ATL
Plasmid#82523PurposeLentivirus vector. Expresses mutant Halotag-Cbx7-delta-ATL fusion proteins in mammalian cells. Cbx7deletion of amino acids 66–83.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationdeletion of amino acids 66–83PromoterCMVAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
CpcBfull-NaGLS fusion_CpcA
Plasmid#74004PurposeExpresses the Nicotiana attenuata GLS as a fusion with the CpcB protein plus the CmR cassete in SynechocystisDepositorInsertNaGLS and CmR
UseTagsExpressionMutationPromotercpcAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJH1307
Plasmid#36910DepositorInsertScLEU2
UseLentiviralTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
SK-YFP-ST1-B
Plasmid#48662PurposeBacterial ST1 repression YFP reporter: protospacer BDepositorInsertST1 prototspacer B/PAM with reporter EYFP
UseCRISPRTagsExpressionMutationPromoterlambda pR promoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
PM-ST1!TA
Plasmid#48653PurposeBacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicolDepositorInsertBacterial ST1 crRNA to prototspacer A
UseCRISPRTagsExpressionMutationPromoterJ23100 promoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
p35SZ
Plasmid#51513PurposepMDC32 with Zif268:FokI followed by approximately 3kb of filler sequenceDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant expression t-dnaTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBACgus_Sumostar/LRRK2 1867-2176
Plasmid#40392DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2176PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus_Sumostar/LRRK2 1867-2161
Plasmid#40391DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pALS3-Ma-sfGFP-WT
Plasmid#212119PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-human histone 4-in-One
Plasmid#223237PurposePolycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4DepositorInsertsHis6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII
UseTagsDual StrepII, His6, TEV protease site, and VSVGExpressionBacterialMutationA103GPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_B_miR-7
Plasmid#220468PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version B)DepositorInsertSUMO_Eu1
UseTags3xHAExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_B_miR-7.M4
Plasmid#220469PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version B)DepositorInsertSUMO_Eu1
UseTags3xHAExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJP_Azul01
Plasmid#219670PurposeBlue light inducible systemDepositorInsertssimilar to T7 RNA polymerase (core)
similar to YF1-fixJ
UseSynthetic BiologyTagsExpressionMutationPromoterLacIq35 and pJ23105Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJP_Ctrl10
Plasmid#219672PurposeContains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.DepositorInsertsmCherry
lacI
UseTagsExpressionMutationPromoterPT3lacO and pRAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0- Gfap-TagIN-mCherry
Plasmid#196490PurposeTo tag endogenous mouse GFAP with mCherryDepositorInsertsmCherry
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBB328
Plasmid#221533PurposePlasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.DepositorInsertsvioABCE
Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
Tetracycline antibiotic cassette from pRK415
UseTagsExpressionBacterialMutationPromoterNone, contains RBS siteAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only