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jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)

Plasmid

#79785

Purpose

genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing pad

Depositor

Anthony Zador

Insert

MAPP-nL

Use

Synthetic Biology; Sindbis virus genomic plasmid

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin

Plasmid

#166603

Purpose

Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-1066 T12 mutant

Plasmid

#46266

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl T12 mutant (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T12 mutant (D974;K975;R976; R978A) autoinhibition…

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-Caspase-4

Plasmid

#183356

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-BSD-tet-mEGFP-MIB1

Plasmid

#140241

Purpose

Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Tet-inducible mEGFP-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb-IRES-eGFP-W

Plasmid

#32519

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Rheb (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-eGFP-attP(bxb)-*BsdR

Plasmid

#183751

Purpose

Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox251

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-P2A-MCS (Neo)

Plasmid

#89181

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCA14-pHR-SFFV-GFP-RPA43

Plasmid

#199445

Purpose

Expression of GFP-RPA43 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

RPA43 (POLR1F Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGFP-P2A-mCherry

Plasmid

#203868

Purpose

Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

Plasmid

#191170

Purpose

EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP (plant)

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP2-P2A-FusionRed

Plasmid

#188734

Purpose

Express miniGFP2 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-hS1pr1-GFP-Neo

Plasmid

#203708

Purpose

Overexpression of FLAG-S1PR1-GFP fusion.

Depositor

Timothy Hla

Insert

Flag-hS1PR1-GFP (S1PR1 Human)

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MscL(G22S)-c-term-cpGFP-ERexp

Plasmid

#195346

Purpose

Control plasmid for the MscL tension biosensor

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

MscL(G22S)-c-term-cpGFP-ERexp

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVd2

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d4EGFP

Plasmid

#41943

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSMPUW-miR-124-GFP-Puro

Plasmid

#117321

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Lentiviral

Tags

GFP-Puro

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Gata3-T2A-H2B-eGFP donor

Plasmid

#113119

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata3 donor region including homology arms and T2A-H2B-eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-eGFP-W3SL

Plasmid

#218742

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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