MscL(G22S)-c-term-cpGFP-ERexp
(Plasmid
#195346)
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PurposeControl plasmid for the MscL tension biosensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePuro
- Backbone size w/o insert (bp) 6100
- Total vector size (bp) 7282
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMscL(G22S)-c-term-cpGFP-ERexp
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SpeciesE. coli
- Promoter CMVd2
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATGAGCATTATTAAAGAATTTCGCGAATTTGCG
- 3′ sequencing primer CACTTCGTTTTCATAGCAAAAGCTGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.10.04.510821v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MscL(G22S)-c-term-cpGFP-ERexp was a gift from Allen Liu (Addgene plasmid # 195346 ; http://n2t.net/addgene:195346 ; RRID:Addgene_195346) -
For your References section:
Mechanosensitive Channel-Based Optical Membrane Tension Reporter. Hsu YY, Resto Irizarry AM, Fu J, Liu AP. ACS Sens. 2023 Jan 27;8(1):12-18. doi: 10.1021/acssensors.2c01921. Epub 2023 Jan 6. 10.1021/acssensors.2c01921 PubMed 36608338