Addgene

Showing: 10101 - 10120 of 14839 results

Results per page:

Sort results by:

pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSuper 44-1

Plasmid

#51597

Purpose

shRNA 1 against rat and mouse Sema4D

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA1

Use

RNAi

Tags

Expression

Mutation

Promoter

U6

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pRS423-SgH

Plasmid

#163972

Purpose

Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SF-1 shRNA #1

Plasmid

#28186

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pSP571

Plasmid

#139498

Purpose

CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD Cas9 / sgRNA (STL162)

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pGPD

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-SMG6-CDS-1

Plasmid

#136046

Purpose

SMG6 shRNA (Targeting CDS #1) inserted into the PLKO.1 plasmid (AACTTGTAAGTAACCTGCAGC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SMG6 (SMG6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-SMG6-CDS-2

Plasmid

#136047

Purpose

SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SMG6 (SMG6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSU6-shTOM40.2

Plasmid

#89882

Purpose

Expresses shRNA against TOM40

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVenus-PICK1-shRNA

Plasmid

#72575

Purpose

Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only
pB-puro-mU6-CNPshRNA2

Plasmid

#126612

Purpose

Expresses shRNA against human CNP under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP ShRNA-2

Use

RNAi

Tags

Expression

Mutation

Promoter

Mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-hsg(M)

Plasmid

#138262

Purpose

Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

sgRNA M

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-H1-Syndecan-1 shRNA (UTR)

Plasmid

#40623

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(UTR) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pLLOXmPOLBeta

Plasmid

#24174

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Jan 22. ():.

Insert

pLLOXmPOLbeta (Polb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

x

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pSuperRetro-puro-RalB 45i

Plasmid

#11122

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalAB siRNA (RALA, RALB Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
5aa SunTag VP64 AP3

Plasmid

#115484

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_dCas9-1x5

Plasmid

#63599

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-retro-puro-shNup93-SpeI

Plasmid

#87331

Purpose

Expression of shRNA targeting at Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pARC (pKD-G3)

Plasmid

#62682

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pATI (pKD-G7)

Plasmid

#62686

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge