Addgene

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-mBmi1

Plasmid

#114317

Purpose

TET-inducible expression of the mBmi-1 protein

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

mBmi1 (Bmi1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLSLGUS

Plasmid

#51517

Purpose

pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GUS
3kb filler sequence

Use

Plant t-dna plasmid

Tags

NLS and flag

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato mouse Rab35 WT

Plasmid

#53514

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-ERas-IH

Plasmid

#15688

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMOS030: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163054

Purpose

ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-GFP-Pat coiled-coil (cc)

Plasmid

#31757

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Patronin (Patronin Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Contains only the coiled-coil domain: aa 535-1457

Promoter

Metallothionein

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pREPK219H

Plasmid

#51492

Purpose

pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Rep/RepA

Use

Plant expression t-dna

Tags

Expression

Mutation

changed lysine 219 to histidine

Promoter

2x35S

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pTol1-uas-mAKT-2a-nlstdTomato

Plasmid

#67706

Purpose

Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

mAKT-2A-nlstdTomato

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pT2-cryR;clecl14aP1Egfp

Plasmid

#90159

Purpose

contains clec14a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aP1Egfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST HRP Rab35 WT

Plasmid

#53498

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

HRP

Expression

Insect

Mutation

Promoter

GAL4

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato mouse Rab35 DN

Plasmid

#53513

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Tags

tdtomato

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pLentiN 18E6no*

Plasmid

#37446

Purpose

Lentiviral expression of HPV 18E6no* in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 18E6no* (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

V44A

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TCF25

Plasmid

#67114

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF25 (TCF25 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pUAST YFP mouse Rab35 WT

Plasmid

#46014

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pXL-CAG-mBirA272-NRX3b

Plasmid

#43921

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

BirA272-NRX3b (birA Synthetic, Human)

Use

Tags

2XFLAG

Expression

Mammalian

Mutation

See comments

Promoter

CAG

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFEB

Plasmid

#67097

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFEB (TFEB Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
907K

Plasmid

#160236

Purpose

FlightN-PipR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

pristinamycin repressor
Flightin promoter

Use

Tags

VP16

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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LIIIalpha F C-D

Plasmid

#54384

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiN 5E6

Plasmid

#37449

Purpose

Lentiviral expression of HPV 5E6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 5E6 (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only