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5aa SunTag VP64 EVD

Plasmid

#115483

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pX330(puro) Rosa26-H3F3B

Plasmid

#73131

Purpose

Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

spCas9

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAIO-AsCpf1

Plasmid

#137846

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1-NLS-FLAG

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shMOCS3_2

Plasmid

#102974

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_2 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shMOCS3_1

Plasmid

#102973

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_1 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF2-3'UTR

Plasmid

#136041

Purpose

UPF2 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CGCGAGGGTTAATCTTCTCTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2 (UPF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRalA-2

Plasmid

#15258

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Use

Tags

HA

Expression

Plant

Mutation

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mutation

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pSicoR human DGCR8-3

Plasmid

#14771

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pPPC009.106

Plasmid

#171142

Purpose

Expression of J106 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J106 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcnab2

Plasmid

#192795

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcnab2

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x3

Plasmid

#58773

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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RAB14 shRNA

Plasmid

#61496

Purpose

to knockdown endogenous RAB14 expression levels

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSico-shNKCC1

Plasmid

#83027

Purpose

expresses an shRNA against NKCC1

Depositor

Angelique Bordey

Article

Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

Insert

shNKCC1

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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PLKO.1-REG1-CDS

Plasmid

#136053

Purpose

REG1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (TATGGGATCAAGTGCCGATTC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

REG1 (ZC3H12A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_2

Plasmid

#102964

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_2 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSuper mouse DGCR8-3

Plasmid

#14774

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only