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pAG414GPD-FvCYP722C2(pYL878)

Plasmid

#178299

Purpose

Expresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

FvCYP722C2

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-OsCYP711A3(pYL761)

Plasmid

#178300

Purpose

Expresses OsCYP711A3 from O. sativa in Saccharomyces cerevisiae. Centromeric TRP, PGPD-OsCYP711A3-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A3

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pID2.02

Plasmid

#26955

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;basPEgfp

Plasmid

#90169

Purpose

contains a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

basalPEgfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 SREBF2

Plasmid

#67129

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SREBF2 (SREBF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMM2_4

Plasmid

#183057

Purpose

HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mRuby2
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mRuby2 and mTurquoise2

Expression

Yeast

Mutation

Promoter

HAA1 and YGP1

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_10

Plasmid

#183059

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Mutation

Promoter

HAA1 and Synthetic promoter

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_11

Plasmid

#183060

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Mutation

Promoter

HAA1 and Synthetic promoter

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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p1177-X1-8

Plasmid

#38150

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2011 May;77(1):53-61

Insert

Carboxypeptidase A

Use

Tags

HHHHHHRR

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 SP6

Plasmid

#67074

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SP6 (SP6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBGRY

Plasmid

#61599

Purpose

Transformation vector pBGRY

Depositor

John Carlson

Article

Koh et al Neuron. 2014 Aug 20;83(4):850-65. doi: 1

Type

Empty backbone

Use

Drosophila transformation vector

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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LIIalpha F 2-3

Plasmid

#54348

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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LIIalpha F 1-2

Plasmid

#54346

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LIIalpha F 4-5

Plasmid

#54352

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LIIalpha R 3-4

Plasmid

#54351

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASp YFP Rab4 CA

Plasmid

#53466

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab04

Use

Tags

YFP

Expression

Insect

Mutation

Q67L

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pTol1-UAS-nlstdTomato

Plasmid

#67693

Purpose

Tol1 expression plasmid with UAS-driven myc tagged nlsTdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

6xMTnlsTomato

Use

Tags

6xMyc-NLS

Expression

Mutation

Promoter

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMEclecl14aP1gfp

Plasmid

#90123

Purpose

Gateway Mid-entry clone containing promoter sequence specific for clec14a driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aP1Egfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHPdestVenus

Plasmid

#24568

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

Venus

Expression

Mutation

Promoter

Available since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pCZGY3195

Plasmid

#172695

Purpose

Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Punc-25

Use

Tags

GFP 1-10

Expression

Worm

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge