p1177-X1-8
(Plasmid
#38150)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFastBac-1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6526
- Total vector size (bp) 6522
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Modifications to backboneGateway cloning cassette was inserted into pFastBac-1 to create pDEST-8.
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCarboxypeptidase A
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Alt nameMeCPA
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SpeciesM. anisopliae
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Insert Size (bp)1278
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GenBank IDEU919684
- Promoter polyhedron
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Tag
/ Fusion Protein
- HHHHHHRR (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer polyhedron_fwd_primer
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p1177-X1-8 was a gift from David Waugh (Addgene plasmid # 38150 ; http://n2t.net/addgene:38150 ; RRID:Addgene_38150) -
For your References section:
The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: insights into their use as tools for the removal of affinity tags. Austin BP, Tozser J, Bagossi P, Tropea JE, Waugh DS. Protein Expr Purif. 2011 May;77(1):53-61. Epub 2010 Nov 10. 10.1016/j.pep.2010.11.005 PubMed 21073956
