Addgene

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-mcs2

Plasmid

#138362

Purpose

Gateway compatible 5' entry clone contains mutli-cloning sites sequence2.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pME GFP-PCNA

Plasmid

#105977

Purpose

entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

PCNA (PCNA Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3-mNG-STOP

Plasmid

#218644

Purpose

Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pME Integrin beta 1b

Plasmid

#105976

Purpose

entry clone for gateway cloning, Full length Integrin beta 1b without stop

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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BRAF - A12G in pENTR

Plasmid

#216606

Purpose

gateway cloning donor vector containing BRAF ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

BRAF (BRAF Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

A12G

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-globin

Plasmid

#82582

Purpose

5' entry vector containing frog translation elongation factor 1 alpha (EF1a) enhancer fused to rabbit b-globin intron for semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

Frog EF1a enhancer fused to rabbit b-globin intron

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPTK002-2-pGAP

Plasmid

#84961

Purpose

Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glyceraldehyde-3-phosphate dehydrogenase promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

BsaI site removed (1805 c to g)

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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