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p3E-CDC42-DN

Plasmid

#109584

Purpose

Multisite gateway vector for 3' tagging with dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

dominant negative

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-puro FLAG wildtype, catalytic active lipin 1

Plasmid

#32010

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD1 (OTU, aa 287-437)

Plasmid

#61407

Purpose

Expresses human OTUD1 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286 and aa 438-481.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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AKT-APEX2-P2A-mKate2

Plasmid

#67654

Purpose

Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH-APEX2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB2 (full-length, aa 1-234)

Plasmid

#61421

Purpose

Expresses human OTUB2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xML1N(7Q)

Plasmid

#67666

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1Nmut*2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6A (OTU, aa 128-288)

Plasmid

#61417

Purpose

Expresses human OTUD6A (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6A (OTUD6A Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-127.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6B (OTU, aa 133-293)

Plasmid

#61419

Purpose

Expresses human OTUD6B (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-132.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas6

Plasmid

#106274

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6-KRAB-2a-BlastR

Plasmid

#126492

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-KRAB-2a-BlastR

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas8e

Plasmid

#106270

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasA

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCse2

Plasmid

#106271

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasB

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas7

Plasmid

#106272

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasC

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas5

Plasmid

#106273

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasD

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (OTU, aa 132-314)

Plasmid

#61410

Purpose

Expresses human OTUD2 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-131 and aa 315-348.

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-ARF1-DN

Plasmid

#109536

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ARF1 (arf1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

DN

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-EEA1

Plasmid

#109560

Purpose

Multisite gateway vector for 5' tagging with early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

early endosome associated 1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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