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Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLOXp-GUS
Plasmid#154059PurposeLevel 0.5 Golden Gate vector, loxP-GUS-nosT-loxPDepositorInsertbeta-glucoronidase (E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF280
Plasmid#167675PurposeIntermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoterpj23119Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-NucGFP
Plasmid#184696PurposeLentiviral transfer plasmid for expression of NucGFPDepositorInsertNucGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKar2-msGFP-HDEL
Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
UseTagsExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC151
Plasmid#118899Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3CDepositorInsertNcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsGST tag, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-2AeGFP
Plasmid#123638PurposeMammalian expression, Genome editingDepositorInsertAsCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BRAT1
Plasmid#62802PurposeExpresses human BRAT1 with an N-terminal HA tag in mammalian cellsDepositorInsertBRAT1 (BRAT1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W
Plasmid#112927PurposeCRISPR-a reporter assay lentiviral vector (gTetO)DepositorInserthU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU53
Plasmid#167679PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRTagsExpressionYeastMutationPromoterpRPR1(TetO)Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU54
Plasmid#167680PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL90002
Plasmid#68261PurposeLevel 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)DepositorInsertPromoter: U6-26 (Arabidopsis thaliana)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-tag
Plasmid#55180PurposeFLAG-tagDepositorInsertFLAG
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRU293
Plasmid#167688PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-mtIF3 shRNA_TagRFP657
Plasmid#182367PurposeshRNA targeting mtIF3 mRNADepositorInsertmtIF3 (Mtif3 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15α1
Plasmid#101712PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-GLICNot-C-His
Plasmid#140187PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTags6xHis and GSTExpressionMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only