1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZmUBQ:GW

   Plasmid

   #132358

   Purpose

   Expression of CDS from the Zea mays ubiquitin promotor

   Depositor

   Paul Schulze-Lefert

   Article

   Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

   Type

   Empty backbone

   Use

   Tags

   YFP

   Expression

   Plant

   Mutation

   Promoter

   pZmUBQ

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113343

   Purpose

   Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   p300core-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHRE

   Plasmid

   #134909

   Purpose

   Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.

   Depositor

   George Lomonossoff

   Article

   Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   double 35S

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SPI1_pet28a

   Plasmid

   #131653

   Purpose

   Bacterial expression of ETS gene SPI1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAC1749

   Plasmid

   #127983

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGS-21a-PcAad1

   Plasmid

   #163039

   Purpose

   expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

   Depositor

   Jean-Marie Francois

   Article

   Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

   Insert

   aryl-alcohol dehydrogenase
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSF280

   Plasmid

   #167675

   Purpose

   Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOCC153

    Plasmid

    #118901

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Htb2-GFP

    Plasmid

    #117669

    Purpose

    Colorize the nuclei

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    Htb2-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the gene encoding the Histone Htb2 from …

    Promoter

    pGAP

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pj23119

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLOXp-GUS

    Plasmid

    #154059

    Purpose

    Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    beta-glucoronidase (E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112927

    Purpose

    CRISPR-a reporter assay lentiviral vector (gTetO)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-AsCas12a-2AeGFP

    Plasmid

    #123638

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    AsCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRU53

    Plasmid

    #167679

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-HA-BRAT1

    Plasmid

    #62802

    Purpose

    Expresses human BRAT1 with an N-terminal HA tag in mammalian cells

    Depositor

    Toru Ouchi

    Article

    So et al BMC Cancer. 2014 Jul 29;14:548. doi: 10.

    Insert

    BRAT1 (BRAT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pOCC151

    Plasmid

    #118899

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    GST tag, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICSL90002

    Plasmid

    #68261

    Purpose

    Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6-26 (Arabidopsis thaliana)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge