Addgene

We narrowed to 5,448 results for: #16

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p4055 HA-E6AP isoform III

Plasmid

#8659

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform III (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Promoter

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pDB007ns2a

Plasmid

#99214

Purpose

Accessory plasmid for PACE of PylRS variants; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)

Use

Tags

Expression

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp, PproK, and Ptet

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

Plasmid

#175521

Purpose

Nuclear expression of HaloTag10 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10

Use

Tags

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-Cre

Plasmid

#73474

Purpose

Expresses Cre for circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

Cre recombinase

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-shSrc

Plasmid

#26982

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Src (SRC Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGS-N2c(G)

Plasmid

#73481

Purpose

N2c Glycoprotein expression vector

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

N2c(G)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only
RabV CVS-N2c(deltaG)-GCaMP6f

Plasmid

#73466

Purpose

Expresses GCaMP6f for calcium imaging

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

GCaMP6f

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Use

Tags

luciferase

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-C2 Chfr (1014)

Plasmid

#61853

Purpose

mammalian expresion of Chfr fused to EGFP

Depositor

Jonathon Pines

Article

Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

Insert

Chfr (CHFR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
pJ3 SHP2 CS

Plasmid

#8319

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

C459S, mutation of essential catalytic cysteine

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pBAD-TRP120

Plasmid

#71732

Purpose

inducible expression of TRP120 in E. coli

Depositor

Jere McBride

Article

Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

Insert

TRP120

Use

Tags

6xHIS and Thioredoxin

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJH2972

Plasmid

#175285

Purpose

Pmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Pmyo-3

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS500 EF1alpha-GFPcre

Plasmid

#11920

Depositor

Brian Sauer

Article

Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

Plasmid

#194934

Purpose

LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b V12

Plasmid

#118323

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b V12 (RAP1B Bovine)

Use

Tags

Flag

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5089

Plasmid

#85125

Purpose

KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiB-kaiB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGC680

Plasmid

#107018

Purpose

GFP reporter of lag-2 driven by 3 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

lag-2

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDB036d

Plasmid

#99216

Purpose

Accessory plasmid for PACE of MjTyrRS variants; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)

Use

Tags

Expression

Bacterial

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp, PproK, and PproA

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge