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Plasmid#129086PurposeExpression of luxB in mammalian cellsDepositorInsertco luxB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BRAF-V600E
Plasmid#116204PurposeLentiviral expression of BRAF V600EDepositorInsertBRAF (BRAF Human)
UseLentiviralTagsExpressionMutationV600EPromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3h
Plasmid#208699PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mCherry-53BP1
Plasmid#127658PurposeOver-express mCherry-53BP1 fragment to monitor 53BP1 foci.DepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsmCherryExpressionMutationNucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…PromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
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YFP-Parkin-IRES-zeo
Plasmid#61728PurposeExpression of human Parkin fused to eYFPDepositorInsertParkin (PRKN Human)
UseTagseYFPExpressionMammalianMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_HaloTag7_T2A_EGFP
Plasmid#169325PurposeExpression of HaloTag7 in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
hLRP1-pEZT-BM
Plasmid#177985PurposeExpression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cellsDepositorInsertLDL receptor related protein 1 (LRP1 Human)
UseTagsNoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonor MCS Rosa26
Plasmid#37200DepositorInsertsRosa26 left targeting arm
MCS
Rosa26 right targeting arm
UseGenomic targeting - zinc fingerTagsExpressionMutationPromoterAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP
Plasmid#20672PurposeDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas ServiceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG HRP-TM
Plasmid#44441DepositorArticleInsertHorseradish peroxidase fused to the transmembrane domain of PDGFR
UseTagsHA tag and myc tagExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K0-P2A-RFP
Plasmid#105686PurposeDouble fluorescent stalling reporter K0DepositorInsertsP2A-3xFLAG-VHPbeta
K0
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-BMTU UDG-6xHis
Plasmid#172197Purposeexpresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactionsDepositorInsertBMTU UDG
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-TNNT2-Neo
Plasmid#214013PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato
Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1
Plasmid#197061PurposeDonor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)DepositorInsertsEncapsulin
Adaptor Protein
UseCRISPRTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2
Plasmid#40260PurposeRFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertmRuby2
UseTagsHis6 and XpressExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-gRNA-Actb1216
Plasmid#155368PurposePspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylationDepositorInsertPspCas13b gRNA targeting Actb 1216
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-G12V-KRAS-IRES-Blast
Plasmid#153336PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cellsDepositorInsertmCherry-G12V-KRAS (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBG
Plasmid#22227PurposeGST fusion vectorDepositorTypeEmpty backboneUseTagsGSTExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 WT
Plasmid#16237DepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE2max-∆RNaseH-dualU6
Plasmid#198735PurposeAAV genome encoding C-terminal PE2max and U6 expression cassettesDepositorInsertNpuC-CtermPE2max∆RNaseH
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS6
Plasmid#55752PurposeProduces T7 RNA Polymerase.DepositorInsertT7 RNA Polymerase
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HDAC6 Flag
Plasmid#13823PurposeMammalian expression of human histone deacetylase 6 with flag tagDepositorInsertHDAC6 (HDAC6 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
TFORF3036
Plasmid#144512PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF4 (ATF4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLGB13
Plasmid#126618PurposeSuicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-Puro (BRD4)
Plasmid#91796PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-Puro
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SC35-cMyc
Plasmid#44721DepositorInsertSC35 (SRSF2 Human)
UseTagshis and mycExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-INSR-V5/HIS
Plasmid#201980Purposeexpression of human INSR receptor tyrosine kinase in mammalian cellsDepositorInsertINSR receptor tyrosine kinase (INSR Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-calpainsensor
Plasmid#36182Purposedetection of activity of ubiquitous calpainsDepositorInsertalpha-Fodrin cleavage site (Sptan1 Mouse)
UseTagseCFP and eYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCXB-EBNA1
Plasmid#41857DepositorInsertEBNA-1 (EBNA-1 human herpesvirus 4)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi
Plasmid#27320DepositorInsertzebrafish ubiquitin promoter (ubb Zebrafish)
UseMultisite gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
R777-E139 Hs.NF1
Plasmid#70423PurposeGateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)DepositorInsertNF1 (NF1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2
Plasmid#31829DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4
Plasmid#26103PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis - TEVExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorInsertstracrRNA
dcas9
CRISPR array
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-mEGFP
Plasmid#129590PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_12x-601_MA1
Plasmid#114358Purpose12x Widom 601 nucleosome sequenceDepositorInsert12X601-MA1
UseUnspecifiedTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPer-Red-C199S
Plasmid#48252PurposeHydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.DepositorInsertHYPER-RED-C199S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-HALOnls
Plasmid#121937PurposeCRISPR-Sirius plasmidDepositorInsertMCP-HALOnls
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MP9956:SFG.GD3synthase-2A-GD2synthase
Plasmid#75013PurposeRetroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.DepositorInsertsmurine GD3 synthase
murine GD2 synthase
UseRetroviral; Momlv retroviral vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#209725PurposeExpresses cytosolic low affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_763
Plasmid#201156PurposeEnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRaDepositorInsert5x-ALFA tag-dEnAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationD908APromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only