Showing: 981 - 1000 of 8204 results
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Plasmid#52254Purposetransient expression of pcoCas9 gene in plant cellsDepositorInsertpcoCas9
UseCRISPR; Plant expressionTagsFLAG and NLSExpressionMutationPromoterhybrid constitutive promoter 35SPPDKAvailabilityAcademic Institutions and Nonprofits only -
pUC119-gRNA
Plasmid#52255PurposeCan use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.DepositorInsertguide RNA targeting AtPDS3 (PDS3 Mustard Weed)
UseCRISPR; Plant expressionTagsExpressionMutationPromoterArabidopsis U6 polymerase III promoterAvailabilityAcademic Institutions and Nonprofits only -
pFGC-pcoCas9
Plasmid#52256PurposeA binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNADepositorInsert35SPPDK:pcoCas9:NOS cassette
UseCRISPR; Plant expressionTagsExpressionMutationPromoterconstitutive 35SPPDK promoterAvailabilityAcademic Institutions and Nonprofits only -
pSUMO1
Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pSUMO2
Plasmid#52259PurposeBacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-flag-YTHDF2
Plasmid#52300Purposefull length YTHDF2 with N-terminal flag tag for mammalian cell expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsflagExpressionMammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1
Plasmid#52326Purposeexpresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationR117Q mutation (R95Q according to numbering in Fi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Left
Plasmid#52341PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Left
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Right
Plasmid#52342PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Right
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-EGFP
Plasmid#52343PurposeAAVS1 donor vector for inducible gene expressionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
AAVS1-CAG-hrGFP
Plasmid#52344PurposeAAVS1 donor vector for constitutive gene expressionDepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
delta PE OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52380PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsUseTagsExpressionMammalianMutationdeleted -1620 - -1011 upstream from OCT4 ATGPromoterAvailabilityAcademic Institutions and Nonprofits only -
mouse deltaPE Oct4-GFP transgenic reporter
Plasmid#52382PurposeBAC recombineering generated construct, used as a reporter for mouse Oct4 expressionDepositorInsertsUseTagsExpressionMammalianMutationdeleted -300 - -1300bp fragment upstream from Oct…PromoterOct4 and PGK+gb2AvailabilityAcademic Institutions and Nonprofits only -
pLXSN16E6E7
Plasmid#52394PurposeThe plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.DepositorInsertHPV16 E6E7
UseRetroviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Kif2A
Plasmid#52401PurposeExpresses GFP-tagged Kif2A in mammalian cells under the CMV promoterDepositorInsertKif2A (KIF2A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p21-Reporter
Plasmid#52432PurposeDNA damage inducible Red Fluorescent Protein for mammalian expressionDepositorInsertp21-p53 Tandem Response Element Promoter
UseTagsTurboRFPExpressionMammalianMutationPromoter13X repeated p21-p53 response elementAvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Calreticulin-N-16
Plasmid#54023PurposeLocalization: Endoplasmic Reticulum, Excitation: 487, Emission: 509DepositorInsertCalreticulin (CALR Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 981 - 1000 of 8204 results