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B. adolescentis mRNA toehold switch sensor

Plasmid

#110710

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis species specific toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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B. adolescentis 16S toehold switch sensor

Plasmid

#110700

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAD02 S. paradoxus telomerase RNA KBS

Plasmid

#61891

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces paradoxus KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Use

Tags

Expression

Mutation

Promoter

T7

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

Plasmid

#129405

Purpose

circular Pepper RNA

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-Pepper(TAR Variant-2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag

Plasmid

#231591

Purpose

Express circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-2xALFATag-10xSunTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-1xALFA-5xSunTag

Plasmid

#231597

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG

Plasmid

#167477

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(K42M)-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

Plasmid

#167476

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(T286A)-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag

Plasmid

#231601

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized

Plasmid

#231602

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag_codon_optimized

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A4

Plasmid

#216823

Purpose

Expresses acyclovir-controllable IRE variant #A4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A4

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8

Plasmid

#216824

Purpose

Expresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3

Plasmid

#216825

Purpose

Expresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4

Plasmid

#216826

Purpose

Expresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-4

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Plasmid

#167478

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Plasmid

#167479

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159501

Purpose

Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159502

Purpose

Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159504

Purpose

Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159506

Purpose

Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge