pAD02 S. paradoxus telomerase RNA KBS
(Plasmid
#61891)
-
PurposePlasmid for run-off in vitro transcription of Saccharomyces paradoxus KBS RNA
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonePuc19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 2760
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTelomerase RNA hairpin
-
Alt nameTLC1 KBS
-
SpeciesSaccharomyces paradoxus
-
Insert Size (bp)89
-
GenBank IDAY639015.1
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Fok1 site follows RNA construct to linearize plasmid prior to in vitro transcription.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAD02 S. paradoxus telomerase RNA KBS was a gift from Thomas Cech (Addgene plasmid # 61891 ; http://n2t.net/addgene:61891 ; RRID:Addgene_61891) -
For your References section:
RNA recognition by the DNA end-binding Ku heterodimer. Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR. RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 Apr 22. 10.1261/rna.038703.113 PubMed 23610127