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Showing: 201 - 250 of 28594 results

  11. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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  12. pMSCV_puro_41589

    Plasmid
    #111632
    Purpose
    Complementation of Dicer_KO mESCs with mutated human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (K70A) (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K70A
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    111632-plasmid-map-sequence-id-217766

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  13. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid
    #98219
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    human synapsin
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98219-plasmid-map-sequence-id-256052

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  17. pLKO.1 puro

    Plasmid
    #8453
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    8453-plasmid-map-sequence-id-421039

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  20. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  22. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74630-plasmid-map-sequence-id-373032

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  34. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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  35. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Article
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    170069-plasmid-map-sequence-id-333408

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  37. pMT-puro

    Plasmid
    #17923
    Purpose
    Insect expression vector
    Depositor
    David Sabatini
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Drosophila metallothionein gene promoter drives e…
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17923-plasmid-map-sequence-id-211593

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  41. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110403-plasmid-map-sequence-id-213570

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  47. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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Showing: 201 - 250 of 28594 results