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Addgene
Showing: 9881 - 9900 of 52901 results

  4. pJ609 Flag-Sumo1

    Plasmid
    #49046
    Purpose
    Mammalian expression of N-terminally tagged Sumo1
    Depositor
    Lianna Swanson
    Insert
    SUMO1 (SUMO1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49046-plasmid-map-sequence-id-72029

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  8. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid
    #49060
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP (chloride-sensitive)
    Expression
    Mammalian
    Mutation
    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49060-plasmid-map-sequence-id-72178

    Enlarge

  9. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49061-plasmid-map-sequence-id-72183

    Enlarge

  10. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid
    #49062
    Purpose
    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    N-terminal deletion of amino acids 13-222 in hNKC…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49062-plasmid-map-sequence-id-72184

    Enlarge

  11. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

    Plasmid
    #49063
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49063-plasmid-map-sequence-id-72185

    Enlarge

  12. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

    Plasmid
    #49064
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49064-plasmid-map-sequence-id-72188

    Enlarge

  13. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

    Enlarge

  14. pcDNA3.1 Flag YFP hNKCC1 C295S (NT103)

    Plasmid
    #49066
    Purpose
    Expresses human NKCC1 C295S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49066-plasmid-map-sequence-id-72192

    Enlarge

  15. pcDNA3.1 Flag YFP hNKCC1 C295A (NT104)

    Plasmid
    #49067
    Purpose
    Expresses human NKCC1 C295A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49067-plasmid-map-sequence-id-72193

    Enlarge

  16. pcDNA3.1 Flag YFP hNKCC1 C543M (NT360)

    Plasmid
    #49068
    Purpose
    Expresses human NKCC1 C543M mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C543M in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49068-plasmid-map-sequence-id-72195

    Enlarge

  17. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49069-plasmid-map-sequence-id-72196

    Enlarge

  18. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid
    #49070
    Purpose
    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    ECL4 deletion of amino acids 562-582 in hNKCC1 in…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49070-plasmid-map-sequence-id-72197

    Enlarge

  19. pcDNA3.1 Flag YFP hNKCC1 C630A (NT399)

    Plasmid
    #49071
    Purpose
    Expresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C630A in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49071-plasmid-map-sequence-id-72198

    Enlarge

  20. pcDNA3.1 Flag YFP hNKCC1 C630S (NT400)

    Plasmid
    #49072
    Purpose
    Expresses human NKCC1 C630S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C630S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49072-plasmid-map-sequence-id-72199

    Enlarge

Showing: 9881 - 9900 of 52901 results