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PurposeExpresses luciferase under control of human insulin gene promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.10[luc2]
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4242
- Total vector size (bp) 4614
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman INS promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)410
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Entrez GeneINS (a.k.a. IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4)
- Promoter INS promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer RVprimer4 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL410_INS421 was a gift from Kevin Ferreri (Addgene plasmid # 49057 ; http://n2t.net/addgene:49057 ; RRID:Addgene_49057) -
For your References section:
Insulin gene expression is regulated by DNA methylation. Kuroda A, Rauch TA, Todorov I, Ku HT, Al-Abdullah IH, Kandeel F, Mullen Y, Pfeifer GP, Ferreri K. PLoS One. 2009 Sep 9;4(9):e6953. doi: 10.1371/journal.pone.0006953. 10.1371/journal.pone.0006953 PubMed 19742322