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pPE3-pPuro

Plasmid

#215722

Purpose

Cloning vector for Uni-Vector prime editing system

Depositor

Kou-Juey Wu

Article

Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

Insert

PE2

Use

Unspecified

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTECH-chAcK3RS(IPYE)

Plasmid

#104069

Purpose

Expression plasmid encoding chAcK3RS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chAcK3RS(IPYE); PproK pyl

Use

Tags

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Use

Tags

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-BRCA1

Plasmid

#99394

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

Depositor

Junjie Chen, Michael Huen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA1 (BRCA1 Human)

Use

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Blast

Plasmid

#207716

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHyo182

Plasmid

#173136

Purpose

Expresses pheS_A294G in e.coli

Depositor

Seokhee Kim

Article

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pheS_A294G

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

Plasmid

#107132

Purpose

cGAL-N driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Use

Tags

Expression

Worm

Mutation

Promoter

C. elegans eft-3 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP 150TAG

Plasmid

#197574

Purpose

Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6)

Use

Tags

His6

Expression

Bacterial

Mutation

N150TAG

Promoter

araC and lpp

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

Plasmid

#107136

Purpose

cGAL-C driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Use

Tags

Expression

Worm

Mutation

Promoter

C. elegans eft-3 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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CD-MPR-mCherry-RUSH

Plasmid

#202798

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-Huwe1

Plasmid

#37431

Depositor

Jean Cook

Insert

Huwe1 (HUWE1 Human)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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ER-J-TAG (pGS730)

Plasmid

#160038

Purpose

pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

Depositor

David Sabatini

Insert

3xHA-sfGFP-JPH-TMD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163476

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV14-3X-Flag-SARS-CoV-2 S

Plasmid

#145780

Purpose

Over expression of SARS-CoV-2 S (spike) in mammalian cell line

Depositor

Zhaohui Qian

Article

Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

Insert

SARS-CoV-2 S (S SARS CoV 2)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Codon optimized and C-terminal 19 amino acids del…

Promoter

CMV

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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AA331

Plasmid

#215954

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1

Plasmid

#36412

Depositor

Benjamin Glick

Type

Empty backbone

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Plasmid

#32702

Purpose

Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

eGFP

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-wt-TTF-1

Plasmid

#49989

Purpose

For transient transfection to express human wild type TTF-1 protein

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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