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Showing: 9921 - 9940 of 152602 results

  2. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  10. 5KO9 (HMCES)

    Plasmid
    #101877
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HMCES (HMCES Human)
    Use
    Tags
    6x His (modified C-term tag is AENLYFQSHHHHHH)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101877-plasmid-map-sequence-id-194533

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  12. p3E_he1a:mCherry

    Plasmid
    #113881
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113881-plasmid-map-sequence-id-223633

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  13. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  15. pCMV-Spike

    Plasmid
    #173921
    Purpose
    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter
    Depositor
    Andreas Stuermer
    Insert
    SARS-2 Spike Protein (S Human, SARS-COV-2, Synthetic)
    Use
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    standard CMV
    Available since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173921-plasmid-map-sequence-id-358849

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  19. ePop

    Plasmid
    #50953
    Purpose
    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations
    Depositor
    Lingchong You
    Article
    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:
    Inserts
    E lysis protein
    LuxI
    LuxR
    Use
    Tags
    Expression
    Mutation
    Early stop codon introduced in mutated luxR resul…
    Promoter
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50953-plasmid-map-sequence-id-454119

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Showing: 9921 - 9940 of 152602 results