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Plasmid#166163PurposeGateway entry vector for N. furzeriDDX5DepositorArticleInsertDDX5
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::eGFP-H11
Plasmid#211941Purposedual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHSP68Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTspan12(1-305)-1D4_pTT5
Plasmid#216381PurposeMammalian expression vector to transiently express full-length human Tspan12 with a Rho1D4 tagDepositorInsertTetraspanin-12 (TSPAN12 Human)
UseTagsRho1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)
Plasmid#218640PurposeGateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationdelPYD (I125M-END)PromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3 R262W (disease-associated mutation)
Plasmid#218647PurposeGateway shuttling vector for cloning NLRP3-R262W (no stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsExpressionMutationR262WPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3(ΔPYD, I125M-END)
Plasmid#218641PurposeGateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationdelPYD (I125M-END)PromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3 (STOP)
Plasmid#218638PurposeGateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
msHook2 g2 lentiCRISPRv2-mCherry
Plasmid#218653PurposeKnockout vector for mouse Hook2DepositorInsertHook2 (Hook2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ecoli PancACe
Plasmid#215712PurposeExpresses acetyl-CoA biosensor in EcoliDepositorInsertPancACe
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionTagsExpressionMutationPromoterT7 for each geneAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-FL-Efg1
Plasmid#216395PurposeExpresses MBP-tagged full length Efg1DepositorInsertEfg1-FL
UseTags6xHis tags-MBPExpressionBacterialMutationPromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD
Plasmid#216396PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1DepositorInsertEfg1-ΔDBD
UseTags6xHis-tagsExpressionBacterialMutationdeleted amino acids 182-356PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-N-PrLD
Plasmid#216397PurposeExpresses 6xHis-tagged N-terminal prion-like domains of Efg1DepositorInsertEfg1-N-PrLD
UseTags6xHis-tagsExpressionBacterialMutationdeleted amino acids 182-554PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-C-PrLD
Plasmid#216398PurposeExpresses 6xHis-tagged C-terminal prion-like domains of Efg1DepositorInsertEfg1-C-PrLD
UseTags6xHis-tagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-DBD
Plasmid#216399PurposeExpresses 6xHis-tagged DBD of Efg1DepositorInsertEfg1-DBD
UseTags6xHis-tagsExpressionBacterialMutationdeleted amino acids 2-181 and 357-554PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC APEX2-3xFLAG-IRES-eGFP
Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mScarlet HDR template
Plasmid#214998PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template
Plasmid#214999PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1050
Plasmid#209540PurposeBTK Type 1 homology arm for Golden Gate assemblyDepositorInsertUpstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1054
Plasmid#209544PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome at a neutral siteDepositorInsertRecombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only