Addgene

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V. natriegens NC7

Bacterial Strain

#215356

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
V. natriegens NC1

Bacterial Strain

#215355

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-knt-P-R

Bacterial Strain

#188475

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-bla-P-R

Bacterial Strain

#188474

Purpose

Cre-inducible activation of bla for ampicillin/carbenicillin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-knt-P*-R

Bacterial Strain

#188476

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-knt-P**-R

Bacterial Strain

#188477

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-knt-P-R*

Bacterial Strain

#188478

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-cat-P-R

Bacterial Strain

#188479

Purpose

Cre-inducible activation of catfor chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-cat-T172A-P-R

Bacterial Strain

#188480

Purpose

Cre-inducible activation of catT172Afor chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-tetA-Ptet-Rtet

Bacterial Strain

#188481

Purpose

Cre-inducible activation of tetA for tetracycline resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
DHIP1

Bacterial Strain

#226851

Purpose

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only
delta guaB

Bacterial Strain

#113653

Purpose

Base strain that the pDCAF plasmids (Addgene #113646-113652) must be in.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Bacterial Resistance

Kanamycin

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits
Akaby

Bacterial Strain

#184224

Purpose

Akaby is a RecB knockout E. coli strain, made for cell-free expression system for linear templates.

Depositor

Kate Adamala

Article

Sato et al PLoS One. 2022 Apr 7;17(4):e0266272. doi

Bacterial Resistance

Kanamycin

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecBCD

Bacterial Strain

#176581

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Jean-Loup Faulon, Chase Beisel

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecBCD

Bacterial Strain

#176583

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecB

Bacterial Strain

#176580

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Jean-Loup Faulon, Chase Beisel

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecB

Bacterial Strain

#176582

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 GamS

Bacterial Strain

#176584

Purpose

E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
bMS.346

Bacterial Strain

#220588

Purpose

Increase editing efficiency of retron and oligo recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 28 results

Results per page:

Sort results by:

V. natriegens NC7

V. natriegens NC1

MG1655-OptoCre-knt-P-R

MG1655-OptoCre-bla-P-R

MG1655-OptoCre-knt-P*-R

MG1655-OptoCre-knt-P**-R

MG1655-OptoCre-knt-P-R*

MG1655-OptoCre-cat-P-R

MG1655-OptoCre-cat-T172A-P-R

MG1655-OptoCre-tetA-Ptet-Rtet

DHIP1

delta guaB

Akaby

BL21 ΔrecBCD

BL21 Rosetta2 ΔrecBCD

BL21 ΔrecB

BL21 Rosetta2 ΔrecB

BL21 Rosetta2 GamS

C321.∆A.opt

bMS.346

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