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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Expression

Mutation

Promoter

hSyn

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

Plasmid

#130998

Purpose

Double floxed ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

ChRmine

Use

AAV

Tags

mScarlet

Expression

Mutation

Promoter

Ef1a

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

Plasmid

#160369

Purpose

Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-3xHA

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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TCARZ-CBh-GFP-SV40PA-GF3-R4A22

Plasmid

#203767

Purpose

For production of template RNA for use in PRINT, GFP under CBh promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

CBh

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pXD054 KIKO CD19 CAR AAV vector

Plasmid

#192157

Purpose

CD19 CAR AAV vector

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

anti-CD19 CAR (CD19 Human)

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

Plasmid

#192190

Purpose

HER2 CAR AAV vector PRODH2 KI (pLY080b)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)

Use

AAV; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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TCARZ-CMV-Cherry-PAmin-GF3-R4A22

Plasmid

#203770

Purpose

For production of template RNA for use in PRINT, mCherry under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for mCherry

Use

Ivt template

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP

Plasmid

#111584

Purpose

An AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrmScarlet-I-P2A-post-eGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

tetO

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-mCardinal-P2A-B19L Flp-in vector

Plasmid

#178519

Purpose

For making TRE-CB mouse line in Tigre locus

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCardinal-P2A-B19L

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

Plasmid

#198508

Purpose

Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

Promoter

CaMKIIα minimal promotor (0.4kb)

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

Plasmid

#203838

Purpose

Flp-dependent Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mScarlet-KASH-2A

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)

Plasmid

#228044

Purpose

Lentiviral stable Inflammasome speck reporter, designed from PMC9623368.

Depositor

Wade Harper

Insert

CASP1[CARD]-mSG2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pXD043 KIKO CD22 CAR AAV vector

Plasmid

#192158

Purpose

CD22 CAR AAV vector

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

anti-CD22 CAR (CD22 Human)

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

Plasmid

#203839

Purpose

Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mScarlet-KASH-2A

Expression

Mutation

Enzymatically inactive Cre

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)

Plasmid

#228043

Purpose

DONR vector for inflammasome speck reporter, designed from PMC9623368.

Depositor

Wade Harper

Insert

CASP1[CARD]-mSG2

Use

Gateway donr

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChRmine-oScarlet

Plasmid

#137160

Purpose

Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChRmine-p2a-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

oScarlet E95D

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

Plasmid

#111925

Purpose

Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183521

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet-Kv2.1

Use

AAV

Tags

Expression

Mutation

H33R

Promoter

CaMKIIa

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130991

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge