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Showing: 1921 - 1960 of 3174 results

  7. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

    Plasmid
    #159088
    Purpose
    LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts
    Depositor
    John Rawls
    Article
    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea
    Inserts
    1.7kb fabp6 promoter (fabp6 Zebrafish)
    eGFP
    polyA
    Use
    Fluorescent reporter
    Tags
    Expression
    Mutation
    Promoter
    1.7kb fabp6
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    159088-plasmid-map-sequence-id-313129

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  10. pCJ199

    Plasmid
    #162672
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162672-plasmid-map-sequence-id-321466

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  11. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid
    #184408
    Purpose
    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD
    Depositor
    Jesse Bloom
    Article
    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1
    Insert
    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    Use
    Tags
    HA and c-Myc
    Expression
    Yeast
    Mutation
    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184408-plasmid-map-sequence-id-362479

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  12. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162670-plasmid-map-sequence-id-321452

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  14. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

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  29. pWZL-Blast FAP S624A

    Plasmid
    #207402
    Purpose
    Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.
    Depositor
    Jing Yang
    Article
    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi
    Insert
    Fibroblast Activation Protein Alpha (FAP Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    changed Serine 624 to Alanine. It's a protea…
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207402-plasmid-map-sequence-id-414821

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  30. pWZL-Blast FAP A657S

    Plasmid
    #207403
    Purpose
    Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.
    Depositor
    Jing Yang
    Article
    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi
    Insert
    Fibroblast Activation Protein Alpha (FAP Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    changed Alanine 657 to Serine. It's an endop…
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207403-plasmid-map-sequence-id-414820

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  40. AAVS1-Hb9-CD14

    Plasmid
    #204344
    Purpose
    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    human CD14 (CD14 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hb9 (Mnx1) 9kb promoter fragment
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    204344-plasmid-map-sequence-id-407654

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Showing: 1921 - 1960 of 3174 results