Addgene

We narrowed to 2,243 results for: hells

Showing: 1441 - 1470 of 2243 results

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pcDNA3.1-Zeo-ssIFNG

Plasmid

#183470

Purpose

Expression of the Salmo salar gamma interferon

Depositor

Bertrand Collet

Article

Collins et al Fish Shellfish Immunol. 2014 Oct;40(2):6

Insert

Salmo salar interferon gamma precursor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0R3K2G2

Plasmid

#163263

Purpose

Inducible expression of UniProt identifier A0A0R3K2G2

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0R3K2G2

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pANG02

Plasmid

#178084

Purpose

Gentamycin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG01 or pANG03.

Depositor

Sören Abel

Article

Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

NA

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PoliIFN-γ

Plasmid

#107289

Purpose

Plasmid can be used for secretion of fish Interferon gamma

Depositor

Bohdan Schneider

Article

Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

Insert

Interferon gamma

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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TrubIFN-γ

Plasmid

#107288

Purpose

Plasmid can be used for secretion of fish Interferon gamma

Depositor

Bohdan Schneider

Article

Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

Insert

Interferon gamma

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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OnilIFN-γ

Plasmid

#107287

Purpose

Plasmid can be used for secretion of fish Interferon gamma

Depositor

Bohdan Schneider

Article

Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

Insert

Interferon gamma

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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PCx34.7IL-pET42

Plasmid

#42915

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 34.7 cytoplasmic loop

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 102-179

Promoter

T7

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pTOPO-rDNA-NTS

Plasmid

#23992

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA NTS

Use

Bacterial cloning - topo

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pBM1

Plasmid

#23094

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3, changed Lysine 9 to Glutamine, and Se…

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pBM3

Plasmid

#23096

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Serine 10 to Aspartate and Lys…

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pBM4

Plasmid

#23097

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone 3 changed Serine 10 to Aspartate and Lysi…

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Tags

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-Halo

Plasmid

#200197

Purpose

Expression for SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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NES-Halo

Plasmid

#200201

Purpose

Expression of 3X HIV NES

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X HIV Rev NES-Halo

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock

Plasmid

#47334

Purpose

full length clock in pCMV10 with 3x flag at the N-terminus

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

clock

Use

Tags

3x Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pXD68Kan2-AphA

Plasmid

#191248

Purpose

RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
+36GFP-LPETG

Plasmid

#71754

Purpose

+36GFP-LPETG for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_(pos36GFP)_6XHis_LPETG

Use

Tags

6XHis and Sortase (LPETG)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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APneg-SNAP25a

Plasmid

#168011

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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DHFR-myc

Plasmid

#20214

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AP-SNAP25

Plasmid

#167995

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNA0306

Plasmid

#169452

Purpose

pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1p

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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AP-VAMP4

Plasmid

#168000

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP4(I75-L91)-T7PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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Vh-HA-3xFRB

Plasmid

#20223

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
U_(pos36GFP)-Cys

Plasmid

#71758

Purpose

U_+36GFP-Cys fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_+36GFP-Cys

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
APT3-VAMP7

Plasmid

#168002

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP7-T3'PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-myc

Plasmid

#20228

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T2098L point mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only