...first Addgene plasmid deposit from Professor Stefan Hell, who won the 2014 Nobel Prize in Chemistry for his...the criteria necessary for studying endosomes, the Hell lab designed their super-resolution pH indicators...

...PMID: 16299477. 3. Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly...similar information References 1. Lichtman JW, Conchello JA. Fluorescence microscopy. Nature methods 2,...2, 910-919 (2005). PubMed PMID: 16299476. 2. Conchello JA, Lichtman JW. Optical sectioning microscopy....

...Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiVIPe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiSSTe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...

...Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics continues...Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid...pAAV-mDlx-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 ...

...can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM...Scientists SHOULD Be Using Twitter" podcast from Hello PhD In this podcast the hosts, Josh and Dan, discuss...

... serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2...

...Farewell 2019, and hello 2020! As we're gearing up to start a new decade, we’re also looking back at ...Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela Abitua Thanks to a team effort...

...matching a relatively easy proposition, explained Michelle Baird, who has created plasmids as a technician...Acknowledgments Thank you to Michael Davidson, Michelle Baird, the Davidson lab scientists, and the FSU...

...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t ...While all viruses have a genome inside a protein shell known as a capsid, some also have a lipid bilayer...

...296, 541-545. Pubmed. A. Ertürk, C.P. Mauch, F. Hellal, F. Förstner, T. Keck,  K. Becker, et al. (2011... Jährlin, C.P. Mauch, C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature...

...assemble a crack team of Addgenies that includes: Michelle, the Senior Scientist who coordinates all kit ...myth. The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but even the movie makers ...

...Lightcycler while being relatively easy to multiplex (Thellwell, 2000). See Table 1 for a comparison of features...j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA; Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl...

... enhancer element Gordon Fishell 83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent...hDlx enhancer element Gordon Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...

...Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2...Control Fishell 213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell 213855...Control Fishell 213936 pAAV_BiPVe4_dTomato_nlsdTomato BiPVe4 dTomato/nlsdTomato Control Fishell 213940 ...Control Fishell 213944 pAAV_BiSSTe4_dTomato_nlsdTomato BiSSTe4 dTomato/nlsdTomato Control Fishell Chemogenetics...Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4...Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3_ChR2_mCherry BiLAMP5e3 Activator ChR2 Fishell 213937 pAAV_BiPVe4... Activator ChR2 Fishell 213941 pAAV_BiPVe3_ChR2_mCherry BiPVe3 Activator ChR2 Fishell 213945 pAAV_BiSSTe4...

...driven by SST interneuron-targeting enhancer Gordon Fishell Viviana Gradinaru 213944-PHPeB pAAV_BiSSTe4_dTomato_nlsdTomato...driven by SST interneuron-targeting enhancer Gordon Fishell Viviana Gradinaru 213941-PHPeB pAAV_BiPVe3_ChR2...driven by PV+ basket cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213940-PHPeB pAAV_BiPVe3_dTomato_nlsdTomato...driven by PV+ basket cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213937-PHPeB pAAV_BiPVe4_ChR2...driven by chandelier cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213936-PHPeB pAAV_BiPVe4_dTomato_nlsdTomato...driven by chandelier cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213915-PHPeB pAAV_BiLAMP5e3_ChR2...by Lamp5 interneuron-targeting enhancer Gordon Fishell Viviana Gradinaru 213914-PHPeB pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato...

...-GFP-Fishell-1 Dlx GFP Control Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Fishell 83894...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hDlx Inhibitor DREADD Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hDlx Activator...83894 pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Control Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control...83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 mDIx Activator Optogenetics Fishell 65014 pAAV-hsyn-Jaws-KGC-GFP-ER2...Cre-dependent Calcium sensor Ting 83899 pAAV-mDlx-GCaMP6f-Fishell-2 mDlx GCaMP6f expression under the control of ...of the mDlx enhancer element Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s...Activator DREADD Fishell 98747 pAAV-FLEX-EGFPL10a EF1a EGFPL10a, Cre-dependent Molecular Tool Heintz , ...

...Mammalian Heller 112615 pEHC Emerald and Cre-ERT2 with MCS for inserting promoter none Mammalian Heller 112616...promoter none Mammalian Heller 112618 pCAG-VHC Venus, Cre-ERT2 CAG Mammalian Heller 112619 pCAG-EHC Emerald...Cre-ERT2 CAG Mammalian Heller 112620 pCAG-mTHC TFP, Cre-ERT2 CAG Mammalian Heller 112621 pCAG-tdTHC tdTomato... with MCS for inserting promoter none Mammalian Heller 112617 ptdTHC tdTomato and Cre-ERT2 with MCS for...tdTomato, Cre-ERT2 CAG Mammalian Heller 112622 pVHC_PGKneoLox2DTA.2 Venus, Cre-ERT2, targeting vector with... with MCS for homology arms Mammalian Heller 112623 pEHC_PGKneoLox2DTA.2 Emerald, Cre-ERT2, targeting ...targeting vector with MCS for homology arms Mammalian Heller 112624 pmTHC_PGKneoLox2DTA.2 TFP, Cre-ERT2, targeting...

...pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx...83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Fishell 99130 pAAV-mDlx-NLS-mRuby2...Dlx dTomato Cre dependent 1, 2, 5, 9, rg* Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH Syn EGFP Cre dependent...

...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...

...next week - be sure to come by booth #851 and say hello! Just in time for this meeting, Addgene is launching...