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TFORF0730
Plasmid#143435PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF518A (ZNF518A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG mEmerald-EB3
Plasmid#168514PurposeExpression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live trackingDepositorInsertEB3 (MAPRE3 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCAGAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GASTM-3-RVG-10-Lamp2b-HA
Plasmid#71295PurposeEncodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGASTM mutated glycosylation motif, HA, Lamp2 sig…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
GluN1 (px330:2-guideCas9)
Plasmid#169426PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEY11
Plasmid#191017PurposeFluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInsertUltrapanneuronal
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN1 TKIT donor
Plasmid#169427PurposeDonor DNA for TKIT knockin of SEP to NR1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-TurboID-V5-CDK13_IDG-K
Plasmid#135267PurposeGateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239A
Plasmid#22219DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, threonine …PromoterAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
HyGCaMP6s
Plasmid#102558PurposeExpression of GCaMP6s in HydraDepositorInsertGCaMP6s
UseHydra expressionTagsActin/GCaMP6s fusion protein (C terminal on backb…ExpressionMutationPromoterAvailable sinceOct. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCG_SARS-CoV2 ORF8 C-V5-tag
Plasmid#179990PurposeMammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-2 ORF8 (ORF8 Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA_ASX
Plasmid#98888PurposePlant expression vector for multigene cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterCaMV35S (enhanced)Available sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD011
Plasmid#116852PurposeExpresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011DepositorInsertsAraC
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAM2
Plasmid#198741PurposepGAPDH-GFP, Geneticin resistanceDepositorInsertGFP
UseAcanthamoeba expressionTagsExpressionMutationPromoterpGAPDHAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184V
Plasmid#106199PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-EGFP-ALDH1A1-Neo
Plasmid#189742PurposeThe construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.DepositorInsertALDH1A1 (ALDH1A1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-RGECO1.0
Plasmid#175813PurposeThe lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.DepositorInsertRGECO1.0
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only
