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A single mutation in the core domain of the lac repressor reduces leakiness.
Gatti-Lafranconi P, Dijkman WP, Devenish SR, Hollfelder F
Microb Cell Fact. 2013 Jul 8;12:67. doi: 10.1186/1475-2859-12-67.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
46394pBAD-LacIOverproduction of LacI
46772pLIGFP_W220FProtein overexpression with the modified LacIWF repressor

Antibodies from Article