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Plasmid#69925Purposemammalian expression of human AICD fused to EGFPDepositorInsertAICD (APP Human)
UseTagsEGFPExpressionMammalianMutation47 aa longPromoterCMVAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST FNIP2
Plasmid#164982PurposeFNIP2 expressing vector for mammalian cellsDepositorInsertFNIP2 (FNIP2 Human)
UseTagsGSTExpressionMammalianMutationPromoterCAGAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide
Plasmid#186991PurposeAAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guideDepositorInsertcas7-11-r1045-gggs-r1122, non-targeting crRNA guide
UseAAVTagsExpressionMutationr1045-gggs-r1122 deletionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW646 - pML1>>
Plasmid#115995Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(D669A)
Plasmid#220461PurposeExpress catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
UseTags3xFLAGExpressionMammalianMutationAspartate 669 to alaninePromoterCMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
STAT3(Y705F)-TAL-Luc
Plasmid#46933PurposeLuciferase Reporter for mutant STAT3DepositorInsertSTAT3(Y705F) (STAT3 Human)
UseLuciferaseTagsFLAGExpressionMutationTyrosine 705 to PhenylalaninePromoterTA LuciferaseAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_gRNA
Plasmid#196139PurposegRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFPDepositorInsertAAVS1 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NSP14-Flag
Plasmid#210346PurposeExpresses SARS-CoV-2 NSP14 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-CHC17KDP
Plasmid#59799PurposeExpresses knockdown-proof human CHC17 tagged with GFP.DepositorInsertCHC17 (CLTC Human)
UseTagsGFPExpressionMammalianMutationSilent mutations in amino acids 61-65 to confer s…PromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP-shTACC3
Plasmid#59356PurposeKnocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-oScarlet
Plasmid#137135PurposeViral expression of oScarletDepositorHas ServiceAAV8InsertoScarlet
UseAAVTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HLA-Flag-natT1R3
Plasmid#113950Purposemammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationresidues 21-852PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rosa26_gRNA
Plasmid#196138PurposegRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2DepositorInsertRosa26 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts1
Plasmid#115871PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NRF2-E79Q
Plasmid#207828PurposeFLAG-tagged NRF2-E79Q driven by CMV promoterDepositorInsertNRF2-E79Q (NFE2L2 Human)
UseLentiviralTagsFLAGExpressionMutationE79QPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G88P3-HA-hM3Dq
Plasmid#213972PurposeAAV vector with G88P3 promoter that can drive robust gene expression in MSNsDepositorInsertG88P3
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3
Plasmid#127354Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and mVenusExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
shYAP1 # 2
Plasmid#42541DepositorInsertYAP1 (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF73-2xCSTREP
Plasmid#136232PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73DepositorInsertORF73
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5-(SIM)10
Plasmid#126923PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.DepositorInsert5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationinternal BamHI site between (SUMO)5 and (SIM)10PromotertacAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only