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Plasmid#190913PurposeAddgene #103871 with 3x FLAG on N-terminal of dCas13DepositorInsert3X FLAG
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mOrange2-CI
Plasmid#196852PurposeExpression of red fluorescent reporter mOrange driven by the strong Beta-actin promoterDepositorInsertmOrange2
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_Δ2_VA
Plasmid#186439PurposeEncodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasΔ4_1_2_VA
Plasmid#186440PurposeEncodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-mut4_1_2_VA
Plasmid#186441PurposeEncodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1_2_VB
Plasmid#186442PurposeEncodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1Δ2_VB
Plasmid#186443PurposeEncodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12(PI)
Plasmid#186448PurposeEncodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)DepositorInsertCas12a (PI)
UseCRISPRTagsExpressionMutationK613A, K617APromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12b
Plasmid#186449PurposeEncodes Cas12b from A. acidoterrestris (type V-B)DepositorInsertCas12b
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas12b(RuvC)
Plasmid#186450PurposeEncodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)DepositorInsertdCas12b
UseCRISPRTagsExpressionMutationE848A, D977APromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12b(PI)
Plasmid#186451PurposeEncodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)DepositorInsertCas12b (PI)
UseCRISPRTagsExpressionMutationR122A, G143PPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE
Plasmid#148623PurposeBacterial Expression of DmRoquin_702-819-del790-812DepositorInsertDmRoquin_702-819-del790-812 (roq Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin-del725-755-del790-812_AE
Plasmid#148608PurposeMammalian Expression of DmRoquin-del725-755-del790-812DepositorInsertDmRoquin-del725-755-del790-812 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE
Plasmid#148622PurposeBacterial Expression of DmRoquin_702-819-del725-755DepositorInsertDmRoquin_702-819-del725-755 (roq Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRoquin-del725-755-del790-812_AE
Plasmid#148591PurposeInsect Expression of DmRoquin-del725-755-del790-812DepositorInsertDmRoquin-del725-755-del790-812 (roq Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1467-1717_AE
Plasmid#148598PurposeInsect Expression of DmNot1_1467-1717DepositorInsertDmNot1_1467-1717 (Not1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_275-376-GB1His6_AG
Plasmid#148784PurposeBacterial Expression of HsNot4_275-376DepositorInsertHsNot4_275-376 (CNOT4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_275-575-GB1His6_AG
Plasmid#148788PurposeBacterial Expression of HsNot4_275-575DepositorInsertHsNot4_275-575 (CNOT4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_377-428-GB1His6_AG
Plasmid#148789PurposeBacterial Expression of HsNot4_377-428DepositorInsertHsNot4_377-428 (CNOT4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_400-424_AG
Plasmid#148790PurposeE. coli of HsNot4_400-424DepositorInsertHsNot4_400-424 (CNOT4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only