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Showing: 1881 - 1920 of 149193 results

  6. pLKO.1 mCherry

    Plasmid
    #128073
    Purpose
    puro exchanged to mCherry
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    no
    Promoter
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128073-plasmid-map-sequence-id-255273

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  9. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

    Plasmid
    #194458
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-CD19 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194458-plasmid-map-sequence-id-384169

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  18. pCAG_iPE-N

    Plasmid
    #214734
    Purpose
    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-N_bGH
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214734-plasmid-map-sequence-id-424998

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  19. pTriEx-mCherry-PA-Rac1

    Plasmid
    #22027
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22027-plasmid-map-sequence-id-138515

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  20. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159275-plasmid-map-sequence-id-312908

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  22. pDONR221-SLC3A2_STOP

    Plasmid
    #161379
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC3A2 (SLC3A2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161379-plasmid-map-sequence-id-349390

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  23. pET28a-SnoopCatcher

    Plasmid
    #72322
    Purpose
    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a-SnoopCatcher
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    changed G842T and D848G of S. pneumoniae adhesin …
    Promoter
    T7
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72322-plasmid-map-sequence-id-137253

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  25. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid
    #207404
    Purpose
    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]
    Depositor
    Alexandros Poulopoulos
    Article
    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10
    Insert
    Cas9-RC
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP-2A
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    207404-plasmid-map-sequence-id-413204

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  27. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6-PspCas13b DR-BbsI-BbsI-polyT
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    103854-plasmid-map-sequence-id-350782

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  29. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

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  34. pMitoTimer

    Plasmid
    #52659
    Purpose
    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo
    Depositor
    Zhen Yan
    Article
    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1
    Insert
    DsRed1-E5
    Use
    Dsred
    Tags
    human cytochrome c oxidase subunit VIII mitochond…
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    52659-plasmid-map-sequence-id-430007

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  38. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Article
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    170103-plasmid-map-sequence-id-332603

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  40. CCR2-DuET

    Plasmid
    #213198
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR2 (CCR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213198-plasmid-map-sequence-id-431752

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Showing: 1881 - 1920 of 149193 results