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Plasmid#48358PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
UseTagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAB085d
Plasmid#79209PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); PtetAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
Plasmid#221080PurposeSEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)DepositorInsertACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Flag-BRCA1
Plasmid#223228PurposeInsect expression construct for Flag-tagged BRCA1 protein production and purificationDepositorInsertBreast Cancer 1 (BRCA1 Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GALT-APEX2
Plasmid#215554PurposeStable, inducible expression in GolgiDepositorInsertAPEX2-NES-GalT-EGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PM-APEX2
Plasmid#215557PurposeStable, inducible expression in plasma membraneDepositorInsertLyn11-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7_CC_Vpx-SIV_WPRE_IVT_Template
Plasmid#216792PurposeTemplate for in vitro transcription of Vpx (SIV).DepositorInsertVpx (SIV)
UseTagsExpressionMammalianMutationPromoterT7Available sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMVd1-NLS-GAL4-iLight2-VP16
Plasmid#219550PurposeNLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight2-VP16
UseAAVTagsExpressionMammalianMutationThe (SAGG)x4 linker between GAL4(1-65) and the PC…PromoterCMVd1Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G12-AkaLuc
Plasmid#219551PurposeAkaLuc expression in mammlian cells. pAAV vector. Reporter plasmid.DepositorInsertAkaLuc
UseAAVTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3362
Plasmid#144838PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYOG (MYOG Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-E2F2
Plasmid#202521PurposeExpressing human E2F2 protein with FLAG-tagDepositorInsertE2F2 (E2F2 Human)
UseTagsFLAG-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgCrebbp#2/Cre
Plasmid#193210PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp geneDepositorInsertsgCrebbp#2 (Crebbp Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-MRPS16-VN172
Plasmid#182375PurposemVenus-based mito-riboBiFC vector (Negative control)DepositorInsertMrps16 (Mrps16 Mouse)
UseTagsVN172ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Flag-FOXM1
Plasmid#153136PurposeExpression of Flag tagged human FOXM1 in mammalian cellsDepositorInserthuman FOXM1 (FOXM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR
Plasmid#111177PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Sp
Plasmid#174726PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCK-mScarlet3_C1
Plasmid#189771PurposeConstruct for labelling plasma membranes in mammalian cells using LCK-mScarlet3DepositorInsertLCK-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW12.lentiguide.GFP
Plasmid#104374PurposeGFP vector for cloning in guidesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-PE7 for IVT
Plasmid#214813PurposeTemplate for in vitro transcription of PE7DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSExpressionMutationPromoterT7 (inactivated)Available sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRepCap6
Plasmid#110770PurposepRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.DepositorInsertAAV6 cap, AAV6 rep genes
UseTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
phMYT1L-N174
Plasmid#66809Purpose2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistanceDepositorInsertMYT1L (MYT1L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH-Halo tag
Plasmid#154144PurposeGateway compatible mammalian expression plasmid for halo tag fusion proteinsDepositorTypeEmpty backboneUseTagsHalo tagExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP
Plasmid#58470PurposeExpresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoterDepositorInsertLifeact
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only