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Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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pEGFP-coilin
Plasmid#36906DepositorInsertcoilin (COIL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-BLM
Plasmid#80070PurposeExpresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)DepositorInsertBLM (BLM Human)
UseTagsGFPExpressionMammalianMutationIf correct ID, the cDNA is from HeLa and has char…PromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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3X ERE TATA luc
Plasmid#11354PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response ElementDepositorInsertERE
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRFP
Plasmid#13032PurposeMammalian expression vector for cloning your gene fused to mRFPDepositorHas ServiceCloning Grade DNAInsertMonomer Red Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-N1
Plasmid#136556PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2
Plasmid#14129DepositorInsertTSC2 (TSC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBPZpGAL4DBDUw
Plasmid#26233DepositorInsertZip-GAL4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#42875PurposeA crRNA expression plasmid for targeting a specific sequence.DepositorInsertCRISPR-BsaI
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Drp1K38A
Plasmid#45161DepositorInsertDrp1 (DNM1L Human)
UseTagsHA (AYPYDVPDY)ExpressionMammalianMutationK38APromoterCMVAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-ADRP
Plasmid#87161PurposeFluorescent protein localized to lipid droplets (LDs)DepositorInsertADRP (PLIN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SV40 LT
Plasmid#13970DepositorInsertSV40 LT (SV40gp6 )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EF1α-EGFP
Plasmid#21320PurposeControl Lentiviral vector with strong EF1a promoter, expresses EGFPDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTRKH2
Plasmid#71312PurposeTheta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR
Plasmid#113348PurposeA sgRNA expression plasmid for genome editing in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hM4D(Gi)-mCherry
Plasmid#50479PurposeGi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoterDepositorHas ServiceAAV5InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only