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Plasmid#87429PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1DepositorInsertTJP1 Homology Arms with mEGFP-linker (TJP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-1:PXN-EGFP
Plasmid#87420PurposeHomology arms and linker-EGFP sequence for C-terminus tagging of human PXNDepositorInsertPXN Homology Arms with linker-EGFP (PXN Human)
UseCRISPR; Donor templateTagslinker-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX335A_hCas9(D10A)_IRF8gRNA1
Plasmid#89719PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA1 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA2
Plasmid#89720PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA2 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-24:MYH10-mEGFP
Plasmid#87428PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10DepositorInsertMYH10 Homology Arms with mEGFP-linker (MYH10 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX335A_hCas9(D10A)_IRF8gRNA4
Plasmid#89722PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA4 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA3
Plasmid#89721PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA3 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorInsertP2A-clover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG
Plasmid#176395PurposeExpresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.DepositorInsertGAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG
Plasmid#176396PurposeExpresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.DepositorInsertGAL1 promoter, SZ1-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
N-cadherin in pCCL-c-MNDU3c-PGK-EGFP
Plasmid#38153DepositorInsertsUseLentiviralTagsExpressionMutationPromoterMNDU3c = LTR promoter for U3 region of myeloproli…Available sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184741Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
UseTagsExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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MAC_N_MED13
Plasmid#111689PurposeMAC-tagged gene expressionDepositorInsertMED13 (MED13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO-SOX9
Plasmid#170703Purposedoxycycline-inducible overexpression of SOX9DepositorInsertSOX9 (SOX9 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
Plasmid#79259PurposeExpresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cellsDepositorInsertDmeIF4G (eIF4G1 Fly)
UseSchneider 2 cell expressionTagsV5-His6 and lambdaN-HAExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only