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Plasmid#204211PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on Q175* (*termination)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G
Plasmid#204210PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on R178* (*termination)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G
Plasmid#204212PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpressionDepositorInsertNK2 homeobox 1 (NKX2-1 Human)
UseLentiviralTagsPoint mutation on I207FExpressionMammalianMutationPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse ZNG1
Plasmid#197399Purposeexpresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)DepositorInsertZNG1 (Cbwd1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1
Plasmid#197395PurposeExpresses SUMO-tagged mouse METAP1DepositorInsertMETAP1 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD
Plasmid#148514PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83DepositorInsertDmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD
Plasmid#148515PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-675DepositorInsertDmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T
Plasmid#147590PurposeInsect Expression of DmThor-CG32016-EDepositorInsertDmThor-CG32016-E (Thor Fly)
UseTagsExpressionInsectMutationDmThor two silent mutations compared to the seque…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T
Plasmid#147591PurposeInsect Expression of DmThor-DmCup_417-1133DepositorInsertDmThor-DmCup_417-1133 (Thor Fly)
UseTagsExpressionInsectMutationThor contains two silent mutations A246C and T321…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T
Plasmid#147592PurposeInsect Expression of DmThor-DmeIF4Ga_687-1666DepositorInsertDmThor-DmeIF4Ga_687-1666 (Thor Fly)
UseTagsExpressionInsectMutationDmThor two silent mutations A246C and T321C compa…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgMroh2b#2/Cre
Plasmid#193220PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b geneDepositorInsertsgMroh2b#2 (Heatr7b2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgMroh2b#1/Cre
Plasmid#193219PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b geneDepositorInsertsgMroh2b#1 (Heatr7b2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335 Slc1a3 nickase 1
Plasmid#129410PurposeCas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 geneDepositorInsertsgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKHH030_sgCtrl#2
Plasmid#174143PurposeLentiviral vector expressing a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g3 (BB36)
Plasmid#139454PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g2 (BB35)
Plasmid#139453PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCUU
Plasmid#159747PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _34-66/myc-His
Plasmid#127495PurposeExpresses myc-tagged _34-66 NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _34-66 (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 _34-66PromoterCMVAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 E57A/myc-His
Plasmid#127497PurposeExpresses myc-tagged NDUFS2/E57A in mammalian cellsDepositorInsertNDUFS2 E57A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 E57APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 T58A/myc-His
Plasmid#127498PurposeExpresses myc-tagged NDUFS2/T58A in mammalian cellsDepositorInsertNDUFS2 T58A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 T58APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only