LentiGuide-Neo-NCOA7g3 (BB36)
(Plasmid #139454)

• Purpose: Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker
• Depositing Lab: Caroline Goujon
• Publication: Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376. doi: 10.1038/s41564-018-0273-9. Epub 2018 Nov 26.

Backbone

• Vector backbone: LentiGuide-Neo
• Backbone manufacturer: Caroline Goujon (Addgene plasmid # 139449)
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR
• gRNA/shRNA sequence: g3-NCOA7 5′-gtctggcacaatagaaaatc
• Species: Synthetic
• Entrez Gene: NCOA7 (a.k.a. ERAP140, ESNA1, NCOA7-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3)
• Promoter: EF-1a / U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (unknown if destroyed)
• 3′ cloning site: BsmBI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Lentiguide-Neo-NCOA7g3.gb
• A portion of this plasmid was derived from a plasmid made by: Caroline Goujon (Addgene plasmid # 139449)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry