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Showing: 921 - 940 of 69271 results

  4. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid
    #195315
    Purpose
    Dox inducible lentiviral Cas9 nickase
    Depositor
    Raj Chari
    Insert
    Cas9-nickase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A nickase
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    195315-plasmid-map-sequence-id-390525

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  5. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  10. fru-T2A-QF2 HDR plasmid

    Plasmid
    #141099
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    Use
    Donor template
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141099-plasmid-map-sequence-id-292289

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  11. pLenti-CMV-GFP-miRE_shTHUMPD3-Blast

    Plasmid
    #135690
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135690-plasmid-map-sequence-id-327708

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  12. pLenti-CMV-GFP-miRE_shSCR-Blast

    Plasmid
    #135688
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135688-plasmid-map-sequence-id-327712

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  13. pBlastR-MCS-LexA-VP16-MiniWhite

    Plasmid
    #165900
    Purpose
    Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165900-plasmid-map-sequence-id-326306

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  14. pMXS-IRES-Blast HA-GPAT4-ΔNs5

    Plasmid
    #173169
    Purpose
    Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site
    Depositor
    Kivanc Birsoy
    Article
    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi
    Insert
    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Synonymous mutations at sgRNA sites, amino acids …
    Promoter
    MMLV
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173169-plasmid-map-sequence-id-341378

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  15. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

    Plasmid
    #171817
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171817-plasmid-map-sequence-id-338126

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  16. pCRISPaint-mScarlet-F-hgp100-BlastR [M1G]

    Plasmid
    #171815
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-hgp100-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171815-plasmid-map-sequence-id-338123

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  18. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

    Plasmid
    #171808
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171808-plasmid-map-sequence-id-337980

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  19. pCRISPaint-mNeon-F-hgp100-BlastR [M1G]

    Plasmid
    #171806
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-F-hgp100-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171806-plasmid-map-sequence-id-337977

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Showing: 921 - 940 of 69271 results