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Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Stx17
Plasmid#45911DepositorInsertSyntaxin 17 (STX17 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iCas9-neo
Plasmid#85400PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequenceDepositorInsertFlag-iCas9-P2A-GFP
UseLentiviralTagsFlag-iCas9-P2A-GFPExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
STAT3-GFP
Plasmid#110495Purposereporter of STAT3 signaling activityDepositorInsert4 repeats of STAT3 binding site M67 at GFP promoter region
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA1 with trimmed attP
Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT447
Plasmid#139387PurposeBile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmRDepositorInsertBile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT423
Plasmid#139382PurposeConstitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetRDepositorInsertConstitutively expressed dCas9, LacI and TetR
UseSynthetic BiologyTagsExpressionMutationPromoterP1Available sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP-ova-C1
Plasmid#163524PurposeExpresses both eGFP and chicken ovalbumin in mammalian cellsDepositorInsertenhanced-GFP-chicken ovalbumin tandem
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT445
Plasmid#139386PurposeaTC inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmRDepositorInsertaTC inducible Nanoluc expression
UseSynthetic BiologyTagsExpressionMutationPromoteraTC inducible promoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT444
Plasmid#139385PurposeIPTG inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmRDepositorInsertIPTG inducible Nanoluc expression,
UseSynthetic BiologyTagsExpressionMutationPromoterIPTG inducible promoterAvailable sinceJuly 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT448
Plasmid#139388PurposeBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT450
Plasmid#139390PurposeBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT492
Plasmid#139397PurposeBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT405
Plasmid#139383PurposeLacZ, pNBU1 backbone with BsaI sites, AmpR, ErmRDepositorInsertLacZ
UseSynthetic BiologyTagsExpressionMutationPromoterNo promoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT406
Plasmid#139384PurposeRPU standard, pNBU1 backbone with BsaI sites, AmpR, ErmRDepositorInsertRPU standard
UseSynthetic BiologyTagsExpressionMutationPromoterNo promoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT493
Plasmid#139398PurposeBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only