Metagenome mining and functional analysis reveal oxidized guanine DNA repair at the Lost City Hydrothermal Field.
Utzman PH, Mays VP, Miller BC, Fairbanks MC, Brazelton WJ, Horvath MP
PLoS One. 2024 May 8;19(5):e0284642. doi: 10.1371/journal.pone.0284642. eCollection 2024. PubMed Article
Utzman PH, Mays VP, Miller BC, Fairbanks MC, Brazelton WJ, Horvath MP
PLoS One. 2024 May 8;19(5):e0284642. doi: 10.1371/journal.pone.0284642. eCollection 2024. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210791 | pkk223_TtMutY | pkk223 with Thiotrichaceae MutY insert |
| 210792 | pkk223_TtMutY_D147N_E46Q | pkk223 with Thiotrichaceae MutY containing D147N and E46Q mutations to reduce catalytic activity |
| 210793 | pkk223_TtMutY_F305A_S306A_H307A | pkk223 with Thiotrichaceae MutY containing F305A, S306A, and H307A mutations to reduce recognition of OG:A |
| 210794 | pkk223_MsMutY_D150N_E49Q | pkk223 with Marinosulfonomonas MutY containing D150N and E49Q mutations to reduce catalytic activity |
| 210795 | pkk223_MsMutY_F302A_T303A_H304A | pkk223 with Marinosulfonomonas MutY containing F302A, T303A, and H304A mutations to reduce recognition of OG:A |
| 210796 | pkk223_MsMutY | pkk223 with Marinosulfonomonas MutY insert |
| 210797 | pkk223_RbMutY | pkk223 with Rhodobacteraceae MutY insert |
| 210798 | pkk223_RbMutY_D146N_E45Q | pkk223 with Rhodobacteraceae MutY containing D146N and E45Q mutations to reduce catalytic activity |
| 210799 | pkk223_RbMutY_F298A_T299A_H300A | pkk223 with Rhodobacteraceae MutY containing F298A, S299A, and H300A mutations to reduce recognition of OG:A |
| 213110 | pkk223_EcMutY | pkk223 with E coli MutY insert |
| 213111 | pkk223_Null | pkk223 backbone plasmid |