Addgene

We narrowed to 17,138 results for: gateway

Showing: 9301 - 9320 of 17138 results

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sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP2

Plasmid

#22577

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP2 (USP2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUD5

Plasmid

#22610

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUD5 (OTUD5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2S

Plasmid

#15807

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2S (UBE2S Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pAF018 p3E-2A-mCerulean-CAAX

Plasmid

#195975

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Article

Kirchgeorg et al Dev Dyn. 2018 Oct;247(10):1146-1159. doi

Insert

2A-mCerulean-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2-IP

Plasmid

#15919

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 2 (Sox2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pET-53-RtcB

Plasmid

#51282

Purpose

E. coli expression vector for E. coli RtcB RNA ligase

Depositor

Jay Hesselberth

Article

Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

Insert

E. coli RtcB

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 RNA Polymerase

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
psgRNAc

Plasmid

#114006

Purpose

express sgRNA, p15A, CRM

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMV-BC0547-luc2

Plasmid

#214665

Purpose

barcoded assay, Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered NFAT element with barcode 0547

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2H

Plasmid

#15752

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2H (UBE2H Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mVenus

Plasmid

#118861

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mVenus

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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slc1a3b:mCD8mCherry

Plasmid

#170207

Purpose

Drives mCD8mCherry expression with the slc1a3b promoter in astrocytes

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

mCD8

Use

Zebrafish

Tags

mCherry

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
Active Wnt1-V5

Plasmid

#43807

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

Wnt1 (WNT1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Tags

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMAX-DEST

Plasmid

#37631

Depositor

Valeri Vasioukhin

Article

Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Type

Empty backbone

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
Flag-HA-OTUB2

Plasmid

#22552

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUB2 (OTUB2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-mCherry

Plasmid

#170519

Purpose

Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-mCherry

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-p14FAST

Plasmid

#50025

Purpose

pENTR vector for reptilian orthoreovirus p14 fusion-associated small transmembrane (FAST) protein

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

p14FAST

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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stblHIF: pLenti-PGK-stblHIF-PGK-Puro

Plasmid

#177202

Purpose

encoding stblHIF under PGK promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

PGK

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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