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Showing: 9261 - 9280 of 13976 results

  2. XXBLM (XXBLMA-c075)

    Plasmid
    #98223
    Purpose
    Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag
    Depositor
    Nicola Burgess-Brown
    Insert
    XXBLMA (BLM Human)
    Use
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    98223-plasmid-map-sequence-id-193299

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  3. DCP2 (DCP2B-c016)

    Plasmid
    #98239
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DCP2B (DCP2 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    98239-plasmid-map-sequence-id-193398

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  4. 5WBN (HDAC6)

    Plasmid
    #101267
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC6 (HDAC6 Human)
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    101267-plasmid-map-sequence-id-193501

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  6. pHBSTC159

    Plasmid
    #124353
    Purpose
    Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    NpuDnaBΔ283 (C1G) mini-intein
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Deletion of 283-residue endonuclease domain, C1G
    Promoter
    STC
    Availability
    Academic Institutions and Nonprofits only
    124353-plasmid-map-sequence-id-242353

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  7. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    Contains D10A and H840A mutations
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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  12. pDB4280

    Plasmid
    #98699
    Purpose
    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    98699-plasmid-map-sequence-id-182830

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  13. pDB4281

    Plasmid
    #98700
    Purpose
    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    98700-plasmid-map-sequence-id-182829

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  15. pMW-PahZ1 KT-1

    Plasmid
    #141134
    Purpose
    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
    Depositor
    Mitch Weiland
    Article
    Bolay et al Journal of Chemical Education
    Insert
    PahZ1
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Not the full-length gene. PahZ1 fragment starts a…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    141134-plasmid-map-sequence-id-308652

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  16. pGBW-m4046288

    Plasmid
    #145386
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 endoRNAse
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 endoRNAse (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Availability
    Industry, Academic Institutions, and Nonprofits
    145386-plasmid-map-sequence-id-287563

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  17. pGBW-m4046447

    Plasmid
    #145387
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Availability
    Industry, Academic Institutions, and Nonprofits
    145387-plasmid-map-sequence-id-323027

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  18. pGBW-m4046416

    Plasmid
    #145388
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF8 protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Availability
    Industry, Academic Institutions, and Nonprofits
    145388-plasmid-map-sequence-id-292486

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  19. pGBW-m4046346

    Plasmid
    #145390
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Availability
    Industry, Academic Institutions, and Nonprofits
    145390-plasmid-map-sequence-id-298322

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  20. pGBW-m4046214

    Plasmid
    #145399
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Availability
    Industry, Academic Institutions, and Nonprofits
    145399-plasmid-map-sequence-id-292546

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Showing: 9261 - 9280 of 13976 results