-
Plasmid#21636DepositorInsertRubicon (1700021K19Rik Mouse)
UseTagsEGFPExpressionMammalianMutation*(see below)PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-CD81-10
Plasmid#55281PurposeLocalization: Tetraspanin/Membrane, Excitation: 399, Emission: 456DepositorInsertCD81 (CD81 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW-CCND1-HA-puro
Plasmid#212670PurposeExpress tagged cyclin D1DepositorInsertCCND1 (CCND1 Human)
UseLentiviralTagsHAExpressionMutationPromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDualEX-SMN2
Plasmid#214431PurposeDual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7DepositorInsertSMN2 EXON 7 (SMN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGM9037
Plasmid#153225PurposeTemplate for PCR amplification of the constant part of guide RNAsDepositorInsertguide RNA constant part, carotenoid marker
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9
Plasmid#80425PurposeUsed for in vitro transcription of SpCas9 mRNA.DepositorInsertSpCas9
UseCRISPRTagsnoneExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin (1-76; T66V, L67N)
Plasmid#110755PurposeBacterial expression of human Ubiquitin (1-76; T66V, L67N)DepositorInsertUBB (UBB Human)
UseTagsExpressionBacterialMutationT66V, L67NPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
dClover2-N1
Plasmid#54538PurposeLocalization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.DepositorTypeEmpty backboneUseTagsdClover2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+36)
Plasmid#199165Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+36)
UseTagsMGHHHHHHGGAExpressionMutationE16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2515
Plasmid#91072PurposeModule B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterAtU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-EbpA_g1
Plasmid#153517PurposesgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertpnisA-sgRNA(EbpA_g1)-dCas9 scaffold
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn1_UBC_Bla
Plasmid#61473Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-10aa-H2B
Plasmid#74258PurposeMammalian expression of N-terminal fusion of mRuby3 to H2B.DepositorInsertH2B
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiX2-PURO-shLKB1-Hu
Plasmid#61242PurposeLentivirus expressing shRNA to human LKB1DepositorInsertLKB1 (STK11 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG2IG-Hmga2
Plasmid#122294PurposeOverexpression for mouse Hmga2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Punc-4c.TIR1
Plasmid#194053PurposeTIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegansDepositorInsertTIR1 (TIR1 Mustard Weed)
UseTagsExpressionWormMutationcodon-optimized for expression in C. elegans; Two…Promoterunc-4cAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40
Plasmid#98927PurposeCre dependent AAV expression of GFP spaghetti monster myc constructDepositorHas ServiceAAV1InsertGFP-smFP-myc
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag-SIRT1
Plasmid#8438DepositorInsertSIRT1 (Sirt1 Mouse)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
p1A
Plasmid#162692PurposeExpresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only