pAGM9037
(Plasmid
#153225)
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PurposeTemplate for PCR amplification of the constant part of guide RNAs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGM9121
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Backbone manufacturerMarillonnet, IPB
- Backbone size w/o insert (bp) 2069
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha ; DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameguide RNA constant part, carotenoid marker
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SpeciesSynthetic
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Insert Size (bp)6158
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGM9037 was a gift from Sylvestre Marillonnet (Addgene plasmid # 153225 ; http://n2t.net/addgene:153225 ; RRID:Addgene_153225) -
For your References section:
High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S. Plant Commun. 2020 Nov 23;2(2):100135. doi: 10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100135 PubMed 33898975