-
Plasmid#144234PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5C (KDM5C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pol
Plasmid#65520PurposeExpresses HBV polymerase in mammalian cellsDepositorInsertHBV polymerase protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-His-IleRS
Plasmid#124112PurposeBacterial expression plasmid for PURE system componentsDepositorInsertIsoleucyl-tRNA ligase (ileS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV6-Bmal-luc
Plasmid#68833PurposeExpression of Bmal1-luc in mammalian cellsDepositorInsertBmal1 regulatory sequences (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi
Plasmid#85455PurposemWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
UseTags6xHisExpressionBacterialMutationD341APromoterTlpA and TlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
VRQR-ABEmax
Plasmid#119811PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9
Plasmid#75381PurposedCas9DepositorInsertSp dCas9
UseLentiviralTagsnoExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB533_2E12LI-sfGFP
Plasmid#167527PurposeH3K27me3-mintbody expression vector for mammalian cellsDepositorInsert2E12LI-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plenti-CMV-MCS-RFP-SV-puro
Plasmid#109377PurposeThis plasmid expresses dsRed2 and puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsdsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OASL-V5 puro
Plasmid#158643PurposeLentiviral expression vector for constitutive expression of V5-tagged OASL.DepositorInsertOASL (OASL Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spRYCas9-ABE-UMax
Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Sstr3-GFP-DEST
Plasmid#49098PurposeExpressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSstr3 (Sstr3 Mouse)
UseTagsGFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Drp1K38A
Plasmid#45161DepositorInsertDrp1 (DNM1L Human)
UseTagsHA (AYPYDVPDY)ExpressionMammalianMutationK38APromoterCMVAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: Venus-iLID-CAAX (from KRas4B)
Plasmid#60411PurposeMammalian Expression of Venus-iLID-CAAXDepositorInsertVenus-iLID-CAAX
UseCre/Lox and LentiviralTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorInsertSIRT3 (SIRT3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hMLN
Plasmid#27079PurposeNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOGDepositorInsertC-MYC, LIN28, NANOG (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX037
Plasmid#167156PurposeMoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.DepositorInsertcaffeic acid cycle genes
UseTagsExpressionPlantMutationPromoterpNOS, p35sAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1
Plasmid#131725PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
GSK3beta (1015)
Plasmid#49491Purposeexpresses human Gsk3betaDepositorInsertGSK3beta (GSK3B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
GRM2-DuET
Plasmid#213301PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRM2 (GRM2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only