-
PurposeExpression of Bmal1-luc in mammalian cells
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 68833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLenti6
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5900
- Total vector size (bp) 10234
-
Vector typeMammalian Expression, Lentiviral, Luciferase
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBmal1 regulatory sequences
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)4300
-
Entrez GeneBmal1 (a.k.a. Arnt3, Arntl, BMAL1b, MOP3, bHLHe5, bmal1b')
-
Tag
/ Fusion Protein
- luciferase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AleI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer unknown
- 3′ sequencing primer lucNRev (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLV6-Bmal-luc was a gift from Steven Brown (Addgene plasmid # 68833 ; http://n2t.net/addgene:68833 ; RRID:Addgene_68833)