Skip to main content
Addgene

pLV6-Bmal-luc
(Plasmid #68833)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68833 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 10234
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmal1 regulatory sequences
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4300
  • Entrez Gene
    Bmal1 (a.k.a. Arnt3, Arntl, BMAL1b, MOP3, bHLHe5, bmal1b')
  • Tag / Fusion Protein
    • luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AleI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer lucNRev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV6-Bmal-luc was a gift from Steven Brown (Addgene plasmid # 68833 ; http://n2t.net/addgene:68833 ; RRID:Addgene_68833)